Sensitive detection of HLA-bound peptides on the tumor surface
T cell interactions with antigens lie at the heart of any immune response. TIGL has developed and provides:
- Detection of antigens, including personal neoantigens, through optimized experimental workflows, mass spectrometry, and integrated data analysis, using clinical tissues samples
Immune-monitoring
The strength of TCR binding to cognate antigen (functional avidity) is strongly correlated with in vivo T cell effector function. TIGL has developed and provides:
- ELISPOT assays and multicolor flow cytometry to investigate antigen-specific T cell responses
TCR reconstruction
TIGL has developed and provides:
- A novel TCR reconstruction pipeline that enables the testing of neoantigen-specific TCRs for functional avidity as an indicator of anti-tumor activity using ELISPOT and intracellular cytokine staining
Staff
Derin B. Keskin, PhD, Immunology Lead Scientist
Anna Tarren, Immunology Research Technician