Single-cell transcriptome sequencing (scRNA-seq)
Single-cell transcriptome analysis is a powerful method for dissecting the cell-type composition of a heterogeneous population. TIGL has developed and provides:
- Experimental methods to analyze single cancer cells, single immune cells from tumor infiltrates, and single immune cells from peripheral blood, including droplet- and plate-based methods for scRNA-seq and targeted RNA analysis
Single-cell TCR sequencing (scTCR-seq)
The emerging technology of single-cell sequencing of TCR transcripts provides paired ⍺- and β-chain information, enabling identification of which TCR binds to a particular antigen. TIGL has developed and provides:
- scTCR-seq protocols for
- Single T cells flow sorted into 384-well plates
- Using droplet microfluidics to process larger numbers of T cells
- A method using the paired TCR⍺ and β sequence information to rapidly clone and express the identified TCRs to functionally test antigen specificity
TCR repertoire analysis on RNA
Analysis of TCR repertoire data is an important element in trying to understand the role of TCR diversity in immune responses. TIGL has developed and provides:
- A protocol that uses small amounts of RNA (100 pg to 100 ng) to determine both ⍺ and β TCR repertoire distributions
Staff
Kenneth Livak, PhD, Technology Lead Scientist
Shuqiang Li, PhD, Technology Senior Scientist
Theodore Huang, Technology Research Technician