Immunogenomic analysis

Diagram that shows a differential gene expression analysis comparing no benefit (NB; n = 22) and clinical benefit (CB; n = 13) groups identified 457 genes upregulated in NB samples
Diagram that shows Single cell transcriptome analysis of CD4+ tumor-associated T cells (n=185), freshly isolated at the time of relapse and neoantigen-reactive CD4+ cells isolated from post-vaccination (week 16) peripheral blood (n=104) in glioblastoma Patient 7

The interdependence of immunology and cancer biology has been strengthened with recent advances in immunogenomic analysis through computational approaches.

We aim to take advantage of the data generated from high-throughput sequencing technologies like NGS to systematically identify neoantigens across cancers, antigen presentation states, biomarkers of response, and genetic and epigenetic states of the tumor and host.

TIGL has developed and provides:

  • Bulk transcriptome analysis
  • Single-cell transcriptome analysis
  • TCR analysis
  • Methylation state and RBS detection
  • Neoantigen prediction

Tumor-specific neoantigens are identified using algorithms to predict which mutated peptides are likely to bind to patient-specific MHC class I molecules. TIGL has refined these prediction algorithms incorporating mass spectrometry-based rules as described in the Detection of antigen-specific immunity section. Analysis of large-scale WES data using this pipeline has led to numerous insights regarding the drivers of immune responses across cancer and treatment settings.

Staff

Jeremy Simon, PhD, Computational Lead Scientist

Chloe Tu, Computational Biologist

Cleo Forman, Computational Biologist

Bohoon Shim, Associate Computational Biologist

Yiren Shao, Computational Biologist

Angie McGraw, Computational Biologist

Publications